Coding

Part:BBa_M33334:Design

Designed by: Christopher Brunson   Group: Stanford BIOE44 - S010   (2010-06-05)


CDS+RBS for Inorganic mercury-sensitive regulatory protein merR coding sequence with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A medium strength RBS had to be added (AAAGAGGAGAAA)


Source

Thermus thermophilus HB27, complete genome GenBank: AE017221.1 773178..773606

References